Experimental sample types range from protein gel bands through affinity-purified proteins with interacting partners to complex cell lysates. The lab has expertise in sample preparation, digestion and clean-up prior to mass spectrometry and can advise as necessary on these important aspects of experimental design. In addition to bottom-up discovery-type experiments, the facility has the capability for quantitative proteomic experiments using peptide label (iTRAQ) or label-free (SWATH) approaches.

Click below to find out more about the mass spectrometers we use.

The TripleTOF 6600 separates peptides by reverse phase chromatography during LC-MS.