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Durham University

Department of Biosciences


Publication details for Dr Gary Sharples

G.J. Sharples & R.G. Lloyd (1991). Location of a mutation in the aspartyl-tRNA synthetase gene of Escherichia coli K12. Mutation Research - Fundamental and Molecular Mechanisms of Mutagenesis 264(3): 93-96.

Author(s) from Durham


A mutation (tls-1) that confers a temperature-sensitive growth phenotype in Escherichia coli was shown by DNA cloning and sequencing to be an allele of aspS, the gene for aspartyl-tRNA synthetase. The mutation, which lies near minute 41 on the genetic map, was located some 2.3 kb from the 5' end of the ruvAB operon. A DNA fragment encoding the carboxy-terminus of AspRS was found to be sufficient to allow growth of a tls-1 strain at the non-permissive temperature.