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Durham University

Department of Biosciences


Publication details for Prof Keith Lindsey

Manghwar, H., Lindsey, K., Zhang, X. & Jin, S. (2019). CRISPR/Cas system: recent advances and prospects for genome editing. Trends in Plant Science 24(12): 1102-1125.

Author(s) from Durham


Genome editing (GE) has revolutionized biological research through the new ability to precisely
edit the genomes of living organisms. In recent years, various GE tools have been explored for
editing simple and complex genomes. The clustered regularly interspaced short palindromic repeats (CRISPR)/Cas9 system has widely been used in GE due to its high efficiency, ease of use,
and accuracy. It can be used to add desirable and remove undesirable alleles simultaneously in
a single event. Here, we discuss various applications of CRISPR/Cas9 in a range of important
crops, compare it with other GE tools, and review its mechanism, limitations, and future possibilities. Various newly emerging CRISPR/Cas systems, including base editing (BE), xCas9, and
Cas12a (Cpf1), are also considered.