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Durham University

Department of Biosciences

Profile

Publication details for Professor Paul Denny

Norcliffe, J.L., Alvarez-Ruiz, E., Martin-Plaza, J.J., Steel, P.G. & Denny, P.W. (2014). The utility of yeast as a tool for cell-based, target-directed high-throughput screening. Parasitology 141(1): 8-16.

Author(s) from Durham

Abstract

Many Neglected Tropical Diseases (NTDs) have recently been subject of increased focus, particularly with relation to high-throughput screening (HTS) initiatives. These vital endeavours largely rely of two approaches, in vitro target-directed screening using biochemical assays or cell-based screening which takes no account of the target or targets being hit. Despite their successes both of these approaches have limitations; for example, the production of soluble protein and a lack of cellular context or the problems and expense of parasite cell culture. In addition, both can be challenging to miniaturize for ultra (u)HTS and expensive to utilize. Yeast-based systems offer a cost-effective approach to study and screen protein targets in a direct-directed manner within a eukaryotic cellular context. In this review, we examine the utility and limitations of yeast cell-based, target-directed screening. In particular we focus on the currently under-explored possibility of using such formats in uHTS screening campaigns for NTDs.