Publication details for Prof CAB JahodaAbaci, Hasan Erbil, Coffman, Abigail, Doucet, Yanne, Chen, James, Jacków, Joanna, Wang, Etienne, Guo, Zongyou, Shin, Jung U., Jahoda, Colin A. & Christiano, Angela M. (2018). Tissue engineering of human hair follicles using a biomimetic developmental approach. Nature Communications 9(1): 5301.
- Publication type: Journal Article
- ISSN/ISBN: 2041-1723 (electronic)
- DOI: 10.1038/s41467-018-07579-y
- Further publication details on publisher web site
- Durham Research Online (DRO) - may include full text
Author(s) from Durham
Human skin constructs (HSCs) have the potential to provide an effective therapy for patients with significant skin injuries and to enable human-relevant drug screening for skin diseases; however, the incorporation of engineered skin appendages, such as hair follicles (HFs), into HSCs remains a major challenge. Here, we demonstrate a biomimetic approach for generation of human HFs within HSCs by recapitulating the physiological 3D organization of cells in the HF microenvironment using 3D-printed molds. Overexpression of Lef-1 in dermal papilla cells (DPC) restores the intact DPC transcriptional signature and significantly enhances the efficiency of HF differentiation in HSCs. Furthermore, vascularization of hair-bearing HSCs prior to engraftment allows for efficient human hair growth in immunodeficient mice. The ability to regenerate an entire HF from cultured human cells will have a transformative impact on the medical management of different types of alopecia, as well as chronic wounds, which represent major unmet medical needs.