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Durham University

Department of Biosciences

Academic Staff

Publication details for Dr Akis (Iakowos) Karakesisoglou

Taranum, S., Sur, I., Muller, R., Lu, W. Rashmi, R.N. Munch, M., Neumann, S., Karakesisoglou, I. & Noegel, A.A. (2012). Cytoskeletal interactions at the nuclear envelope mediated by Nesprins. International Journal of Cell Biology 2012: 736524.

Author(s) from Durham

Abstract

Nesprin-1 is a giant tail-anchored nuclear envelope protein composed of an N-terminal F-actin binding domain, a long linker region formed by multiple spectrin repeats and a C-terminal transmembrane domain. Based on this structure, it connects the nucleus to the actin cytoskeleton. Earlier reports had shown that Nesprin-1 binds to nuclear envelope proteins emerin and lamin through C-terminal spectrin repeats. These repeats can also self-associate. We focus on the N-terminal Nesprin-1 sequences and show that they interact with Nesprin-3, a further member of the Nesprin family, which connects the nucleus to the intermediate filament network. We show that upon ectopic expression of Nesprin-3 in COS7 cells, which are nearly devoid of Nesprin-3 in vitro, vimentin filaments are recruited to the nucleus and provide evidence for an F-actin interaction of Nesprin-3 in vitro. We propose that Nesprins through interactions amongst themselves and amongst the various Nesprins form a network around the nucleus and connect the nucleus to several cytoskeletal networks of the cell.