Publication details for Prof A WalmsleySong, L., Rosen, B.P., Borges-Walmsley, M.I. & Walmsley, A. (2002). Evidence for Cooperativity between the Four Binding Sites of Dimeric ArsD, an As (III)-responsive Transcriptional Regulator. Journal of Biological Chemistry 277(29): 25992-26002.
- Publication type: Journal Article
- ISSN/ISBN: 0021-9258, 1083-351X
- DOI: 10.1074/jbc.M201619200
- Further publication details on publisher web site
- Durham Research Online (DRO) - may include full text
Author(s) from Durham
ArsD is a trans-acting repressor of the arsRDABC operon that confers resistance to arsenicals and antimonials in Escherichia coli. It possesses two-pairs of vicinal cysteine residues, Cys12-Cys13 and Cys112-Cys113, that potentially form separate binding sites for the metalloids that trigger dissociation of ArsD from the operon. However, as a homodimer it has four vicinal cysteine pairs. Titration of the steady-state fluorescence of ArsD with metalloids revealed positive cooperativity, with a Hill coefficient of 2, between these sites. Disruption of the Cys112-Cys113 site by mutagenesis of arsD, but not the Cys12-Cys13 site, largely abolished this cooperativity, indicative of interactions between adjacent Cys112-Cys113 sites within the dimer. The kinetics of metalloid binding were determined by stopped flow spectroscopy; the rate increased in a sigmoidal manner, with a Hill coefficient of 4, indicating that the pre-steady-state measurements reported cooperativity between all four sites of the dimer rather than just the intermolecular interactions reported by the steady-state measurements. The kinetics of Sb(III) displacement by As(III) revealed that the metalloid-binding sites behave differentially, with the rapid exchange of As(III) for Sb(III) at one site retarding the release of Sb(III) from the other sites. We propose a model involving the sequential binding and release of metalloids by the four binding sites of dimeric ArsD, with only one site releasing free metalloids.