We use cookies to ensure that we give you the best experience on our website. You can change your cookie settings at any time. Otherwise, we'll assume you're OK to continue.

Department of Mathematical Sciences

Seminar Archives

On this page you can find information about seminars in this and previous academic years, where available on the database.

Statistics Seminars: A statistical base-caller for the Illumina high-throughput DNA sequencing platform

Presented by Wally Gilks, University of Leeds

22 January 2010 14:15 in CM105

We propose statistical base-calling methodology for use with the Illumina Genome Analyzer, a high-throughput next-generation DNA sequencing platform. This methodology takes account of cross-talk between dye labels and three problems which accrue in clusters of DNA sequence over successive cycles of the sequencer: accumulation of dye reactants (the "sticky T" problem); base-incorporation errors (the "phasing" problem); and terminal failure of the sequencing reactions (the problem of "drop-off"). Our methodology allows for differential rates of phase inaccuracy and drop-off between clusters, and calculates the probability of miscall for each base called. The resulting base-calling algorithm is linear in the number of cycles. We evaluate the performance of this algorithm, and compare its base-calling accuracy to that of the Illumina pipeline.

Host: Peter Craig

Contact for more information