Publication details for Dr Elizabeth BromleyBromley, EHC & Hopkinson, I (2002). Confocal microscopy of a dense particle system. Journal Of Colloid And Interface Science 245(1): 75-80.
- Publication type: Journal Article
- ISSN/ISBN: 0021-9797
Author(s) from Durham
We report experiments utilizing confocal microscopy to determine the
position of cornflour starch granules in a paste, as a function of
distance from a wall. The granules have an average diameter of 15 mum.
If a solvent is chosen such as to approximately match the refractive
index of the granules then images of them can be obtained to a depth of
120 mum, which is the limit imposed by the working distance of the x40
oil immersion lens used in the experiment. An algorithm is presented
which successfully identifies the particle centers in 3D. In addition
to experiments on static systems we show how measurements of particle
number density and velocity can be obtained from images of a system in
pipe flow. We find that particles are depleted from the wall over a
range of around 100 mum. (C) 2002 Elsevier Science.