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Durham University

Department of Physics

Staff profile

Publication details for Dr. Cyril Bourgenot

Bourgenot, Cyril, Saunter, Christopher D., Taylor, Jonathan M., Girkin, John M. & Love, Gordon D. (2012). 3D adaptive optics in a light sheet microscope. Optics Express 20(12): 13252-13261.

Author(s) from Durham

Abstract

We report on a single plane illumination microscope (SPIM) incorporating adaptive optics in the imaging arm. We show how aberrations can occur from the sample mounting tube and quantify the aberrations both experimentally and computationally. A wavefront sensorless approach was taken to imaging a green fluorescent protein (GFP) labelled transgenic zebrafish. We show improvements in image quality whilst recording a 3D “z–stack” and show how the aberrations come from varying depths in the fish.