Publication details for Dr Robert PalCarr, Rachel, Puckrin, Robert, McMahon, Brian K., Pal, Robert, Parker, David & Pålsson, Lars-Olof (2014). Induced circularly polarized luminescence arising from anion or protein binding to racemic emissive lanthanide complexes. Methods and Applications in Fluorescence 2(2): 024007.
- Publication type: Journal Article
- ISSN/ISBN: 2050-6120 (electronic)
- DOI: 10.1088/2050-6120/2/2/024007
- Further publication details on publisher web site
- Durham Research Online (DRO) - may include full text
Author(s) from Durham
A circularly polarized luminescence (CPL) spectrometer has been built and used to study the binding interaction of lactate and four different proteins with racemic EuIII and TbIII complexes in aqueous solution. Lactate binding gives rise to strong induced CPL spectra, and the observed emission dissymmetry factors vary linearly with enantiomeric composition. Particularly strong induced TbIII CPL also characterizes the binding interaction of alpha-1-acid glycoprotein with a dissociation constant, Kd, of 2.5 μM.