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Durham University

Department of Chemistry

Publication details for Dr Robert Pal

Ward, E. N. & Pal, R. (2019). Holographic projection for multi-spot structured illumination microscopy. Journal of Microscopy 274(2): 114-120.

Author(s) from Durham

Abstract

This paper describes the design and testing of a multi‐spot structured illumination microscopy system using computer‐generated holograms to create the required excitation patterns. Furthermore, it demonstrates the use of an adapted direct search algorithm for calculating the holograms that allows for imaging across an extended field of view. The system was tested on fixed targets and live cells yielding a two times resolution increase over conventional diffraction‐limited imaging.