Publication details for Dr Robert PalWard, E. N. & Pal, R. (2019). Holographic projection for multi-spot structured illumination microscopy. Journal of Microscopy 274(2): 114-120.
- Publication type: Journal Article
- ISSN/ISBN: 0022-2720 (print)
- DOI: 10.1111/jmi.12787
- Further publication details on publisher web site
- Durham Research Online (DRO) - may include full text
Author(s) from Durham
This paper describes the design and testing of a multi‐spot structured illumination microscopy system using computer‐generated holograms to create the required excitation patterns. Furthermore, it demonstrates the use of an adapted direct search algorithm for calculating the holograms that allows for imaging across an extended field of view. The system was tested on fixed targets and live cells yielding a two times resolution increase over conventional diffraction‐limited imaging.