Publication details for Dr Robert PalWard, Edward N., Torkelsen, Frida H. & Pal, Robert (2018). Enhancing multi-spot structured illumination microscopy with fluorescence difference. Royal Society Open Science 5(3): 171336.
- Publication type: Journal Article
- ISSN/ISBN: 2054-5703 (electronic)
- DOI: 10.1098/rsos.171336
- Further publication details on publisher web site
- Durham Research Online (DRO) - may include full text
Author(s) from Durham
Structured illumination microscopy is a super-resolution technique used extensively in biological research. However, this technique is limited in the maximum possible resolution increase. Here we report the results of simulations of a novel enhanced multi-spot structured illumination technique. This method combines the super-resolution technique of difference microscopy with structured illumination deconvolution. Initial results give at minimum a 1.4-fold increase in resolution over conventional structured illumination in a low-noise environment. This new technique also has the potential to be expanded to further enhance axial resolution with three-dimensional difference microscopy. The requirement for precise pattern determination in this technique also led to the development of a new pattern estimation algorithm which proved more efficient and reliable than other methods tested.