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Durham University

Department of Chemistry

Publication details for Dr Robert Pal

Ward, Edward N., Torkelsen, Frida H. & Pal, Robert (2018). Enhancing multi-spot structured illumination microscopy with fluorescence difference. Royal Society Open Science 5(3): 171336.

Author(s) from Durham


Structured illumination microscopy is a super-resolution technique used extensively in biological research. However, this technique is limited in the maximum possible resolution increase. Here we report the results of simulations of a novel enhanced multi-spot structured illumination technique. This method combines the super-resolution technique of difference microscopy with structured illumination deconvolution. Initial results give at minimum a 1.4-fold increase in resolution over conventional structured illumination in a low-noise environment. This new technique also has the potential to be expanded to further enhance axial resolution with three-dimensional difference microscopy. The requirement for precise pattern determination in this technique also led to the development of a new pattern estimation algorithm which proved more efficient and reliable than other methods tested.