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Durham University

Department of Chemistry

Publication details for Dr Robert Pal

Pal, Robert & Beeby, Andrew (2014). Simple and versatile modifications allowing time gated spectral acquisition, imaging and lifetime profiling on conventional wide-field microscopes. Methods and Applications in Fluorescence 2(3): 037001.

Author(s) from Durham

Abstract

An inverted microscope has been adapted to allow time-gated imaging and spectroscopy to be carried out on samples containing responsive lanthanide probes. The adaptation employs readily available components, including a pulsed light source, time-gated camera, spectrometer and photon counting detector, allowing imaging, emission spectroscopy and lifetime measurements. Each component is controlled by a suite of software written in LabVIEW and is powered via conventional USB ports.