Publication details for Dr Robert PalPal, Robert & Beeby, Andrew (2014). Simple and versatile modifications allowing time gated spectral acquisition, imaging and lifetime profiling on conventional wide-field microscopes. Methods and Applications in Fluorescence 2(3): 037001.
- Publication type: Journal Article
- ISSN/ISBN: 2050-6120
- DOI: 10.1088/2050-6120/2/3/037001
- Further publication details on publisher web site
Author(s) from Durham
An inverted microscope has been adapted to allow time-gated imaging and spectroscopy to be carried out on samples containing responsive lanthanide probes. The adaptation employs readily available components, including a pulsed light source, time-gated camera, spectrometer and photon counting detector, allowing imaging, emission spectroscopy and lifetime measurements. Each component is controlled by a suite of software written in LabVIEW and is powered via conventional USB ports.