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Durham University

Department of Chemistry

Publication details for Dr Robert Pal

McMahon, Brian K., Pal, Robert & Parker, David (2013). A bright and responsive europium probe for determination of pH change within the endoplasmic reticulum of living cells. Chemical communications 49(47): 5363-5365.

Author(s) from Durham

Abstract

A ratiometric EuIII complex has been developed that localises selectively within the endoplasmic reticulum of living cells. Careful calibration, using a time-gated spectral imaging microscope, allows the intensity ratio of emission bands and the variation of excited state lifetime to be used for pH determination, with a pKa of 7.15.