We use cookies to ensure that we give you the best experience on our website. You can change your cookie settings at any time. Otherwise, we'll assume you're OK to continue.

Durham University

Department of Chemistry

Publication details for Dr Robert Pal

McMahon, Brian K., Pal, Robert & Parker, David (2013). A bright and responsive europium probe for determination of pH change within the endoplasmic reticulum of living cells. Chemical communications 49(47): 5363-5365.

Author(s) from Durham


A ratiometric EuIII complex has been developed that localises selectively within the endoplasmic reticulum of living cells. Careful calibration, using a time-gated spectral imaging microscope, allows the intensity ratio of emission bands and the variation of excited state lifetime to be used for pH determination, with a pKa of 7.15.