Publication details for Dr Robert PalMcMahon, Brian K., Pal, Robert & Parker, David (2013). A bright and responsive europium probe for determination of pH change within the endoplasmic reticulum of living cells. Chemical communications 49(47): 5363-5365.
- Publication type: Journal Article
- ISSN/ISBN: 1359-7345, 1364-548X
- DOI: 10.1039/c3cc42308e
- Further publication details on publisher web site
Author(s) from Durham
A ratiometric EuIII complex has been developed that localises selectively within the endoplasmic reticulum of living cells. Careful calibration, using a time-gated spectral imaging microscope, allows the intensity ratio of emission bands and the variation of excited state lifetime to be used for pH determination, with a pKa of 7.15.