Cookies

We use cookies to ensure that we give you the best experience on our website. You can change your cookie settings at any time. Otherwise, we'll assume you're OK to continue.

Durham University

Institute of Medieval and Early Modern Studies (IMEMS)

Staff and Governance

Core Staff

The day-to-day running of IMEMS is the responsibility of the Core Executive Committee, comprising the Director and Associate Directors and the Administrator. 

Publication details for Prof. Andrew Beeby

Hirvonen, Liisa M., Petrášek, Zdeněk, Beeby, Andrew & Suhling, Klaus (2015). Sub-μs time resolution in wide-field time-correlated single photon counting microscopy obtained from the photon event phosphor decay. New Journal of Physics 17(2): 023032.

Author(s) from Durham

Abstract

Fast frame rate complementary metal–oxide–semiconductor cameras in combination with photon counting image intensifiers can be used for microsecond resolution wide-field fluorescence lifetime imaging with single photon sensitivity, but the time resolution is limited by the camera exposure time. We show here how the image intensifier's P20 phosphor afterglow can be exploited for accurate timing of photon arrival well below the camera exposure time. By taking ratios of the intensity of the photon events in two subsequent frames, photon arrival times were determined with 300 ns precision with 18.5 μs frame exposure time (54 kHz camera frame rate). Decays of ruthenium and iridium-containing compounds with around 1 μs lifetimes were mapped with this technique, including in living HeLa cells, using excitation powers below 0.5 μW. Details of the implementation to calculate the arrival time from the photon event intensity ratio are discussed, and we speculate that by using an image intensifier with a faster phosphor decay to match a higher camera frame rate, photon arrival time measurements on the nanosecond time scale could be possible.


Full Executive Committee

Our Full Executive Committee is made up of the Core Executive Committee, listed above, plus a number of executive members including:


International Advisory Board

We are extremely fortunate to have be able to call on the help and guidance of colleagues from around the world who help to shape and guide our direction, strategy and international reach. Our current Advisory Board members are: