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Durham University

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Publication details for Professor Ari Sadanandom

Orosa-Puente, Beatriz, Leftley, Nicola, von Wangenheim, Daniel, Banda, Jason, Srivastava, Anjil K., Hill, Kristine, Truskina, Jekaterina, Bhosale, Rahul, Morris, Emily, Srivastava, Moumita, Kümpers, Britta, Goh, Tatsuaki, Fukaki, Hidehiro, Vermeer, Joop E. M., Vernoux, Teva, Dinneny, José R., French, Andrew P., Bishopp, Anthony, Sadanandom, Ari & Bennett, Malcolm J. (2018). Root branching toward water involves posttranslational modification of transcription factor ARF7. Science 362(6421): 1407-1410.

Author(s) from Durham


Plants adapt to heterogeneous soil conditions by altering their root architecture. For example, roots branch when in contact with water using the hydropatterning response. We report that hydropatterning is dependent on auxin response factor ARF7. This transcription 5 factor induces asymmetric expression of its target gene LBD16 in lateral root founder cells on the side of the root in contact with water. This differential expression pattern is regulated by post-translational modification of ARF7 with the SUMO protein. SUMOylation negatively regulates ARF7 DNA binding activity. ARF7 SUMOylation is required to recruit the Aux/IAA repressor protein IAA3. Blocking ARF7 SUMOylation disrupts IAA3 10 recruitment and hydropatterning. We conclude that SUMO-dependent regulation of auxin response controls root branching pattern in response to water availability.