We use cookies to ensure that we give you the best experience on our website. You can change your cookie settings at any time. Otherwise, we'll assume you're OK to continue.

Durham University

Research & business

View Profile

Publication details for Dr Akis (Iakowos) Karakesisoglou

Padmakumar, V. C., Libotte, T., Lu, W. S., Zaim, H., Abraham, S., Noegel, A. A., Gotzmann, J., Foisner, R. & Karakesisoglou, I. (2005). The inner nuclear membrane protein Sun1 mediates the anchorage of Nesprin-2 to the nuclear envelope. Journal of Cell Science 118(15): 3419-3430.

Author(s) from Durham


Nesprins form a novel class of nuclear envelope-anchored
spectrin-repeat proteins. We show that a direct association
of their highly conserved C-terminal luminal domain with
the inner nuclear membrane protein Sun1 mediates their
nuclear envelope localisation. In Nesprin-1 and Nesprin-2
the conserved C-terminal amino acids PPPX are essential
for the interaction with a C-terminal region in Sun1. In
fact, Sun1 is required for the proper nuclear envelope
localisation of Nesprin-2 as shown using dominant-negative
mutants and by knockdown of Sun1 expression. Sun1 itself
does not require functional A-type lamins for its
localisation at the inner nuclear membrane in mammalian
cells. Our findings propose a conserved nuclear anchorage
mechanism between Caenorhabditis elegans and mammals
and suggest a model in which Sun1 serves as a ‘structural
bridge’ connecting the nuclear interior with the actin