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Durham University

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Publication details for Dr David R.W. Hodgson

David Hodgson (2020). TRIPHOSPHORYLATION REACTION. WO 2020/008209A1. Application filed: 5 July 2019. Granted: 9 January 2020

Author(s) from Durham


The disclosure provides a method of producing a triphosphate compound. The method comprises (a) dissolving imidazole in an aqueous alkaline solution to make an imidazole solution; (b) dissolving phosphoryl halide in an organic solvent to make an organic solution; (c) contacting the imidazole solution and the organic solution to produce a solution comprising phosphorylated imidazole (PIm); (d) removing the organic solvent from the solution comprising PIm; and (e) contacting the PIm with a mutant of nucleoside diphosphate kinase (NDPK) and a diphosphate compound, and thereby producing a triphosphate compound.