Cookies

We use cookies to ensure that we give you the best experience on our website. You can change your cookie settings at any time. Otherwise, we'll assume you're OK to continue.

Durham University

Department of Chemistry

Publication details for Prof. Andrew Beeby

Bryden, Francesca, Maruani, Antoine, Rodrigues, João M. M., Cheng, Miffy H. Y., Savoie, Huguette, Beeby, Andrew, Chudasama, Vijay & Boyle, Ross W. (2018). Assembly of High-Potency Photosensitizer–Antibody Conjugates through Application of Dendron Multiplier Technology. Bioconjugate Chemistry 29(1): 176-181.

Author(s) from Durham

Abstract

Exploitation of photosensitizers as payloads for antibody-based anticancer therapeutics offers a novel alternative to the small pool of commonly utilized cytotoxins. However, existing bioconjugation methodologies are incompatible with the requirement of increased antibody loading without compromising antibody function, stability, or homogeneity. Herein, we describe the first application of dendritic multiplier groups to allow the loading of more than 4 porphyrins to a full IgG antibody in a site-specific and highly homogeneous manner. Photophysical evaluation of UV–visible absorbance and singlet oxygen quantum yields highlighted porphyrin–dendron 14 as the best candidate for bioconjugation; with subsequent bioconjugation producing a HER2-targeted therapeutic with average loading ratios of 15.4:1. In vitro evaluation of conjugate 18 demonstrated a nanomolar photocytotoxic effect in a target cell line, which overexpresses HER2, with no observed photocytotoxicity at the same concentration in a control cell line which expresses native HER2 levels, or in the absence of irradiation with visible light.