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Durham University

Department of Biosciences

Profile

Publication details for Dr Gary Sharples

Sharples, Gary J., Heddle, Jonathan G., Kozak, Maciej, Taube, Michał, Hughes, Timothy R, Pålsson, Lars-Olof, Bowers, Laura Y., Curtis, Fiona A., Plewka, Jacek, Świątek, Sylwia, Paterson, Joy R., Jolma, Arttu, Yang, Ally W.H., Gittens, William H., Trotter, Alexander J., Balakrishnan, Dhanasekaran & Chakraborti, Soumyananda (2020). A bacteriophage mimic of the bacterial nucleoid-associated protein Fis. Biochemical Journal 477(7): 1345-1362.

Author(s) from Durham

Abstract

We report the identification and characterization of a bacteriophage λ-encoded protein, NinH. Sequence homology suggests similarity between NinH and Fis, a bacterial nucleoid-associated protein involved in numerous DNA topology manipulations, including chromosome condensation, transcriptional regulation and phage site-specific recombination. We find that NinH functions as a homodimer and is able to bind and bend double-stranded DNA in vitro. Furthermore, NinH shows a preference for a 15 bp signature sequence related to the degenerate consensus favored by Fis. Structural studies reinforced the proposed similarity to Fis and supported identification of residues involved in DNA binding which were demonstrated experimentally. Overexpression of NinH proved toxic and this correlated with its capacity to associate with DNA. NinH is the first example of a phage-encoded Fis-like nucleoid-associated protein that likely influences phage excision-integration reactions or bacterial gene expression.