Professor John A. Gatehouse, M.A., D.Phil. (Oxon.)
(email at firstname.lastname@example.org)
University of Durham (Department of Botany; Department of Biological Sciences; School of Biological and Biomedical Sciences):
Postdoctoral Research Assistant 1976-1979;
Addison Wheeler Research Fellow 1979-1984;
Currently Deputy Head of School and Director of Undergraduate Teaching
Two main areas of research are currently being pursued; Plant-Insect Interactions, and Recombinant Proteins.
Plant-Insect Interactions, and developing insect-resistant crops, form a multi-disciplinary research programme carried out in collaboration with the group of Prof. Angharad Gatehouse IRES, University of Newcastle, with Dr. Elaine Fitches at CSL York, and with other collaborators, both national and international. The programme has involved the study of both plant and insect biochemistry and molecular biology, to characterise the interaction between plants and their predators at the molecular level. Much current research involves the design and production of recombinant fusion proteins as novel insecticides. These proteins are based on plant lectins as carriers for a variety of toxins, including components from venoms of spiders, scorpions and parasitoid wasps. The research group has also been involved in the production and bioassay of insect-resistant transgenic plants, and in evaluating the effects of these plants on non-target organisms. We believe that genetically modified (GM) crops will make a valuable contribution to future agriculture, and, despite the negative image that has been falsely attributed to them, will come to be seen as an environmentally benign form of pest control.
The research group has extensive experience in the production of recombinant proteins in functional form, using various expression systems, but specialising in the yeast Pichia pastoris as an expression host. These proteins have been used as as starting points for mutagenesis programmes designed to produce novel proteins, for use as catalysts, and in pharmaceutical applications.
- Durham Centre for Crop Improvement Technology
- Molecular Plant Sciences
- Biochemistry and molecular biology of plant-insect interactions
- Expression of recombinant plant and insect proteins in microorganisms
- Genetic engineering of plants for insect resistance
- Insect biochemistry and molecular biology
- Plant biochemistry and molecular biology
- Protein engineering
Indicators of Esteem
- 2006: Invited Speaker: Invited speaker for symposium on “Insect Parasitoids: from basic research to applications”, as part of International Congress of Insect Biotechnology and Industry, Korea, Aug. 2007
Chapter in book
- Gatehouse, J.A. (2013). Genetic Engineering of Crops for Insect Resistance. In Sustainable Food Production. Christou, P., Savin, R., Costa-Pierce, B.A. & Whitelaw, C.B.A. New York: Springer. 808-845.
- Powell, Michelle, Pyati, Prashant, Cao, Min, Bell, Howard, Gatehouse, John A. & Fitches, Elaine (2017). Insecticidal effects of dsRNA targeting the Diap1 gene in dipteran pests. Scientific Reports 7(1): 15147.
- Nakasu, Erich YT, Karamaouna, Filitsa, Partsinevelos, Georgios K, Abd El Halim, Hesham M, Fitches, Elaine C, Pyati, Prashant, Gatehouse, John A, Gatehouse, Angharad MR & Edwards, Martin G (2016). Sublethal effects of the insecticidal fusion protein ω-ACTX-Hv1a/GNA on the parasitoidEulophus pennicornisvia its hostLacanobia oleracea. Pest Management Science 72(3): 585-590.
- Yang, S., Fitches, E., Pyati, P. & Gatehouse, J.A. (2015). Effect of insecticidal fusion proteins containing spider toxins targeting sodium and calcium ion channels on pyrethroid-resistant strains of peach-potato aphid (Myzus persicae). Pest Management Science 71(7): 951-956.
- Yang, S., Pyati, P., Fitches, E. & Gatehouse, J.A. (2014). A recombinant fusion protein containing a spider toxin specific for the insect voltage-gated sodium ion channel shows oral toxicity towards insects of different orders. Insect Biochemistry and Molecular Biology 47: 1-11.
- Price, D.R.G. & Gatehouse, J.A. (2014). Genome-wide annotation and functional identification of aphid GLUT-like sugar transporters. BMC Genomics 15: 647.
- Nakasu, Y.E.T., Williamson, S.M., Edwards, M.G., Fitches, E.C., Gatehouse, J.A., Wright, G. & Gatehouse, A.M.R. (2014). Novel biopesticide based on a spider venom peptide shows no adverse effects on honeybees. Proceedings of the Royal Society B: Biological Sciences 281(1787): 20140619.
- Nakasu, Erich Y.T., Edwards, Martin G., Fitches, Elaine C., Gatehouse, John A. & Gatehouse, Angharad M.R. (2014). Transgenic plants expressing -ACTX-Hv1a and snowdrop lectin (GNA) fusion protein show enhanced resistance to aphids. Frontiers in Plant Science 5: 673.
- Fitches, E.C., Pyati, P., King, G.F. & Gatehouse, J.A. (2012). Fusion to snowdrop lectin magnifies the oral activity of insecticidal omega-Hexatoxin-Hv1a peptide by enabling its delivery to the central nervous system. PLoS ONE 7(6): e39389.
- Pyati, Prashant, Chellamuthu, Anitha, Gatehouse, Angharad M. R., Fitches, Elaine & Gatehouse, John A. (2012). Insecticidal activity of wheat Hessian fly responsive proteins HFR-1 and HFR-3 towards a non-target wheat pest, cereal aphid (Sitobion avenae F.). Journal of Insect Physiology 58(7): 991-999.
- Ferry, N., Stavroulakis, S., Guan, W., Davison, G.M., Bell, H.A., Weaver, R.J., Down, R.E., Gatehouse, J.A. & Gatehouse, A.M.R. (2011). Molecular interactions between wheat and cereal aphid (Sitobion avenae): Analysis of changes to the wheat proteome. PROTEOMICS 11(10): 1985-2002.
- Pyati, P., Bandani, A.R., Fitches, E. & Gatehouse, J.A. (2011). Protein digestion in cereal aphids (Sitobion avenae) as a target for plant defence by endogenous proteinase inhibitors. Journal of Insect Physiology 57(7): 881-891.
- Fang, Q, Wang, F, Gatehouse, J.A., Gatehouse, A.M.R., Chen, X-X, Hu, C & Ye, G-Y (2011). Venom of Parasitoid, Pteromalus puparum, Suppresses Host, Pieris rapae, Immune Promotion by Decreasing Host C-Type Lectin Gene Expression. PLoS ONE 6(10): e26888.
- Hinchliffe, G, Bown, DP, Gatehouse, JA & Fitches, E (2010). Insecticidal activity of recombinant avidin produced in yeast. Journal of Insect Physiology 56(6): 629-639.
- Price, DRG, Tibbles, K, Shigenobu, S, Smertenko, A, Russell, CW, Douglas, AE, Fitches, E, Gatehouse, AMR & Gatehouse, JA (2010). Sugar transporters of the major facilitator superfamily in aphids; from gene prediction to functional characterization. Insect Molecular Biology 19(S2): 97-112.
- Pyati, PS, Bell, HA, Fitches, E, Price, DRG, Gatehouse, AMR & Gatehouse, JA (2009). Cathepsin L-like cysteine proteinase (DcCathL) from Delia coarctata (wheat bulb fly): Basis of insecticidal activity. Insect Biochemistry and Molecular Biology 39(8): 535-546.
- Gatehouse, JA (2008). Biotechnological prospects for engineering insect-resistant plants. Plant Physiology 146(3): 881-887.
- Price, DRG & Gatehouse, JA (2008). RNAi-mediated crop protection against insects. Trends in Biotechnology 26(7): 393-400.
- Trung, NP, Fitches, E & Gatehouse, JA (2006). A fusion protein containing a lepidopteran-specific toxin from the South Indian red scorpion (Mesobuthus tamulus) and snowdrop lectin shows oral toxicity to target insects. BMC Biotechnology 6: 18.
- Mehlo, L, Gahakwa, D, Nghia, PT, Loc, NT, Capell, T, Gatehouse, JA, Gatehouse, AMR & Christou, P (2005). An alternative strategy for sustainable pest resistance in genetically enhanced crops. Proceedings Of The National Academy Of Sciences Of The United States Of America 102(22): 7812-7816.
- Fitches, E, Edwards, MG, Mee, C, Grishin, E, Gatehouse, AMR, Edwards, JP & Gatehouse, JA (2004). Fusion proteins containing insect-specific toxins as pest control agents: snowdrop lectin delivers fused insecticidal spider venom toxinto insect haemolymph following oral ingestion. Journal Of Insect Physiology 50(1): 61-71.
- Fitches, E, Audsley, N, Gatehouse, JA & Edwards, JP (2002). Fusion proteins containing neuropeptides as novel insect control agents: snowdrop lectin delivers fused allatostatin to insect haemolymph following oral ingestion. Insect Biochemistry And Molecular Biology 32(12): 1653-1661.
- Gatehouse, JA (2002). Plant resistance towards insect herbivores: a dynamic interaction. New Phytologist 156(2): 145-169.