Publication details for Dr Arto MaattaSevilla, LM, Nachat, R, Groot KR, Klement, JF, Uitto, J, Djian, P, Määttä, A & Watt FM (2007). Mice deficient in involucrin, envoplakin and periplakin have a defective epidermal barrier. The Journal of Cell Biology 179(7): 1599-1612.
- Publication type: Journal Article
- ISSN/ISBN: 0021-9525, 1540-8140
- DOI: 10.1083/jcb.200706187
- Further publication details on publisher web site
- Durham Research Online (DRO) - may include full text
Author(s) from Durham
The cornified envelope is assembled from transglutaminase cross-linked proteins and lipids in the outermost epidermal layers and is essential for skin barrier function. Involucrin, envoplakin, and periplakin form the protein scaffold on which the envelope assembles. To examine their combined function, we generated mice deficient in all three genes. The triple knockouts have delayed embryonic barrier formation and postnatal hyperkeratosis (abnormal accumulation of cornified cells) resulting from impaired desquamation. Cornified envelopes form but are ultrastructurally abnormal, with reduced lipid content and decreased mechanical integrity. Expression of proteases is reduced and the protease inhibitor, serpina1b, is highly upregulated, resulting in defective filaggrin processing and delayed degradation of desmoglein 1 and corneodesmosin. There is infiltration of CD4+ T cells and a reduction in resident γδ+ T cells, reminiscent of atopic dermatitis. Thus, combined loss of the cornified envelope proteins not only impairs the epidermal barrier, but also changes the composition of T cell subpopulations in the skin.