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School of Biological and Biomedical Sciences

Academic Staff

Publication details for Dr AJ O'Sullivan

Way, G. , Morrice, N. Smythe, C. & O'Sullivan, A.J. (2002). Purification and identification of secernin, a novel cytosolic protein that regulates exocytosis in mast cells. Molecular Biology of the Cell 13(9): 3344-3354.
  • Publication type: Journal papers: academic
  • ISSN/ISBN: 1059-1524
  • DOI: 10.1091/mbc.E01-10-0094
  • Keywords: Permeabilized cells, Hematopoietic-cells, Chromaffin cells, Membrane-fusion, Streptolysin-O, Kinase-C, Secretion, RAC.
  • View online: Online version
  • Durham research online: DRO record

Author(s) from Durham

Abstract

After permeabilization with the pore-forming toxin streptolysin-O mast cells can be triggered to secrete by addition of both calcium and a GTP analogue. If stimulation is delayed after permeabilization, there is a progressive decrease in the extent of secretion upon stimulation, eventually leading to a complete loss of the secretory response. This loss of secretory response can be retarded by the addition of cytosol from other secretory tissues, demonstrating that the response is dependent on a number of cytosolic proteins. We have used this as the basis of a bioassay to purify Secernin 1, a novel 50-kDa cytosolic protein that appears to be involved in the regulation of exocytosis from peritoneal mast cells. Secernin 1 increases both the extent of secretion and increases the sensitivity of mast cells to stimulation with calcium.