Staff profile

Dr Elizabeth Bromley

Personal web page

Lecturer in the Department of Physics

Telephone: +44 (0) 191 33 43644

Room number: 109

Member of the Biophysical Sciences Institute

Member of the Centre for Materials Physics

(email at e.h.c.bromley@durham.ac.uk)

Research Interests

My research centres on the biomolecular design approach to synthetic biology. Current directions include:

The design of a novel protein based motor that walks along DNA.
The design of sequences that adopt multiple native states.
Peptide based methods for coupling changes in chemical potential to mechanical outputs.
Understanding the energy landscape of coiled-coil oligomerization.
The design of self-assembling fibre systems.

For further information please see here.

Research Groups

Centre for Materials Physics

Publications

Articles: magazine

Fletcher, JM, Boyle, AL, Bruning, M, Bartlett, GJ, Vincent, TL, Zaccai, NR, Armstrong, CT, Bromley, EHC, Booth, PJ, Brady, RL, Thomson, AR & Woolfson, DN (2012). A Basis Set of de Novo Coiled-Coil Peptide Oligomers for Rational Protein Design and Synthetic Biology. ACS Synthetic Biology 1(6): 240-250.

Journal papers: academic

Boyle, AL, Bromley, EHC, Bartlett, GJ, Sessions, RB, Sharp, TH, Williams, CL, Curmi, PMG, Forde, NR, Linke, H & Woolfson, DN (2012). Squaring the Circle in Peptide Assembly: From Fibers to Discrete Nanostructures by de Novo Design. Journal of the American Chemical Society 134(37): 15457–15467.
Fonseca, F., Arthur, C. J., Bromley, E. H. C., Samyn, B., Moerman, P., Saavedra, M. J., Correia, A. & Spencer, J. (2011). Biochemical Characterization of Sfh-I, a Subclass B2 Metallo-beta-Lactamase from Serratia fonticola UTAD54. Antimicrobial Agents and Chemotherapy 55(11): 5392-5395.
Fonseca, F., Bromley, E. H. C., Saavedra, M. J., Correia, A. & Spencer, J. (2011). Crystal Structure of Serratia fonticola Sfh-I: Activation of the Nucleophile in Mono-Zinc Metallo-beta-Lactamases. Journal of Molecular Biology 411(5): 951-959.
Xiong, X. L., Bromley, E. H. C., Oelschlaeger, P., Woolfson, D. N. & Spencer, J. (2011). Structural insights into quinolone antibiotic resistance mediated by pentapeptide repeat proteins: conserved surface loops direct the activity of a Qnr protein from a Gram-negative bacterium. Nucleic Acids Research 39(9): 3917-3927.
Samii, L., Blab, G. A., Bromley, E. H. C., Linke, H., Curmi, P. M. G., Zuckermann, M. J. & Forde, N. R. (2011). Time-dependent motor properties of multipedal molecular spiders. Physical Review E 84(3): 031111.
Kuwada, N. J., Zuckermann, M. J., Bromley, E. H. C., Sessions, R. B., Curmi, P. M. G., Forde, N. R., Woolfson, D. N. & Linke, H. (2011). Tuning the performance of an artificial protein motor. Physical Review E: Statistical, nonlinear, and soft matter physics 84(3): 031922.
Bromley, E. H. C., Channon, K. J., King, P. J. S. Mahmoud, Z. N., Banwell, E. F., Butler, M. F., Crump, M. P., Dafforn, T. R. Hicks, M. R., Hirst, J. D., Rodger, A. & Woolfson, D. N. (2010). Assembly Pathway of a Designed α-Helical Protein Fiber. Biophysical Journal 98(8): 1668-1676.
Bromley, EHC, Sessions, RB, Thomson, AR & Woolfson, DN (2009). Designed alpha-Helical Tectons for Constructing Multicomponent Synthetic Biological Systems. Journal Of The American Chemical Society 131(3): 928-930.
Bulheller, BM, Rodger, A, Hicks, MR, Dafforn, TR, Serpell, LC, Marshall, KE, Bromley, EHC, King, PJS, Channon, KJ, Woolfson, DN & Hirst, JD (2009). Flow Linear Dichroism of Some Prototypical Proteins. Journal Of The American Chemical Society 131(37): 13305-13314.
Armstrong, CT, Boyle, AL, Bromley, EHC, Mahmoud, ZN, Smith, L, Thomson, AR & Woolfson, DN (2009). Rational design of peptide-based building blocks for nanoscience and synthetic biology. Faraday Discussions 143: 305-317.
Bromley, EHC, Kuwada, NJ, Zuckermann, MJ, Donadini, R, Samii, L, Blab, GA, Gemmen, GJ, Lopez, BJ, Curmi, PMG, Forde, NR, Woolfson, DN & Linke, H (2009). The Tumbleweed: towards a synthetic protein motor. Hfsp Journal 3(3): 204-212.
Papapostolou, D, Bromley, EHC, Bano, C & Woolfson, DN (2008). Electrostatic control of thickness and stiffness in a designed protein fiber. Journal Of The American Chemical Society 130(15): 5124-5130.
Gribbon, C, Channon, KJ, Zhang, WJ, Banwell, EF, Bromley, EHC, Chaudhuri, JB, Oreffo, ROC & Woolfson, DN (2008). MagicWand: A single, designed peptide that assembles to stable, ordered alpha-helical fibers. Biochemistry 47(39): 10365-10371.
Bromley, EHC, Channon, K, Moutevelis, E & Woolfson, DN (2008). Peptide and protein building blocks for synthetic biology: From programming biomolecules to self-organized biomolecular systems. ACS Chemical Biology 3(1): 38-50.
Channon, K, Bromley, EHC & Woolfson, DN (2008). Synthetic biology through biomolecular design and engineering. Current Opinion In Structural Biology 18(4): 491-498.
Bromley, EHC, Krebs, MRH & Donald, AM (2006). Mechanisms of structure formation in particulate gels of beta-lactoglobulin formed near the isoelectric point. European Physical Journal E 21(2): 145-152.
Rogers, SS, Krebs, MRH, Bromley, EHC, van der Linden, E & Donald, AM (2006). Optical microscopy of growing insulin amyloid spherulites on surfaces in vitro. Biophysical Journal 90(3): 1043-1054.
Bromley, EHC, Krebs, MRH & Donald, AM (2005). Aggregation across the length-scales in beta-lactoglobulin. Faraday Discussions 128: 13-27.
Keetch, CA, Bromley, EHC, McCammon, MG, Wang, N, Christodoulou, J & Robinson, CV (2005). L55P transthyretin accelerates subunit exchange and leads to rapid formation of hybrid tetramers. Journal Of Biological Chemistry 280(50): 41667-41674.
Krebs, MRH, Bromley, EHC & Donald, AM (2005). The binding of thioflavin-T to amyloid fibrils: localisation and implications. Journal Of Structural Biology 149(1): 30-37.
Krebs, MRH, Bromley, EHC, Rogers, SS & Donald, AM (2005). The mechanism of amyloid spherulite formation by bovine insulin. Biophysical Journal 88(3): 2013-2021.
Bromley, EHC & Hopkinson, I (2002). Confocal microscopy of a dense particle system. Journal Of Colloid And Interface Science 245(1): 75-80.