INTERNAL USERS GUIDE

We require 5μl of DNA sample and 3μl of custom primer if required (at a concentration of 3.2pmol/μl) for each sequencing reaction requested.  We hold many universal primers in stock, should these be required.  Please see Primer Information page for more details.

DNA samples should be accurately quantified before submission.  Inaccurate quantification will have a direct impact on the quality of data returned, so we cannot stress enough how important this stage is.  We recommend that samples are run on agarose gel against a known concentration standard (we can help with this initially).  One microlitre of DNA sample should be run, and if not visible then the concentration is likely to be too low for sequencing.  Highly concentrated samples should be diluted and checked again to ensure accuracy.  If unsure about the concentration a labelled gel photograph may be provided, where 1μl of each sample has be run along with a concentration standard. 

Samples should be submitted along with your completed Requisition Form.  Sequence data is normally available the day following submission, and will be uploaded to the file server Pelican.  You will normally only be contacted by us if there is a problem with your samples or data.

Accessing the file server Pelican

From Windows PCs, map a network drive or add a Network Place using the address \\pelican.dur.ac.uk\sequencing\results

Mac users will be able to access the folder at smb://pelican.dur.ac.uk/sequencing/results

If you already have a username and password for Pelican you will be able to sign in using these, otherwise use the guest username SBBS\dna and the password Results 1

You should find a folder named 'Results', inside which are folders for each research group.  Your data will be located in the appropriate folder.  Further information of Pelican can be found at http://www.dur.ac.uk/biological.sciences/local/staff/server/  Any problems accessing the server should be directed to Andrew Hardisty, on extension 41324.