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School of Biological and Biomedical Sciences

Leica SP5

Lens NA
10x HCX PL APO CS 0.4
20x HCX PL APO Oil UV 0.7
40x HCX PL APO Oil UV 1.25
63x HCX PL APO Oil UV 1.4

Leica SP5 Confocal Laser Scanning Microscope SP5

This system has no dichroic mirrors or filters. The laser lines are switched very rapidly by an AOBS (acoustic optical beam splitter) and instead of filters the emitted light is split in to its constituent wavelengths and discriminated by a system of a prism and adjustable spectrometers/mirrors which allows for your emission settings to be completely tunable. In addition our SP5 has 5 PMTs allowing you to images 5 different colours/fluorophores simultaneously. This system is not only capable of high quality images, but can also acquire at speed. The resonance scanner scans at up to 16000 Hz resulting in the ability to produce a 512x512 image at 25 fps, very fast for a point scanner. If you only need to image a smaller slice then this can increase to 250 fps.The microscope in enclosed in a environmental chamber  allowing the control of CO2 and temperature.

This system is good for: Live cellimaging, high quality, high speed.  

Laser lines : 405 / 458 / 478 / 488 / 494 / 514 / 543 / 594 / 633

Filters: Fully tunable